《植物生理学报》 2012, 48(4): 381-385

烈香杜鹃的离体培养和高效植株再生

顾地周*, 陆爽, 巴春影, 李媛媛

通化师范学院生物系, 吉林通化134002

通信作者：顾地周；E-mail: gudizhou@163.com；Tel: 0435-3208073

摘　要：

以烈香杜鹃嫩茎为外植体, 应用均匀设计法筛选其最适合的嫩茎基部直接再生芽苗和生根培养基。结果表明, 最适合烈香杜鹃嫩茎基部直接再生芽苗的诱导培养基为DR+2.30 mg·L-1 TDZ+0.05 mg·L-1 IAA+1.80 mg·L-1 KT, 诱导率为99.6%;生根培养基为1/2DR+0.07 mg·L-1 IAA+0.02 mg·L-1 NAA, 生根率达99.7%以上。以再生植株茎节为材料进行快速繁殖, 在35d的培养周期内, 每段增殖倍数平均达5以上。

关键词：烈香杜鹃; 离体培养; 植株再生; 均匀设计

收稿：2011-12-20   修定：2012-02-19

资助：吉林省科技厅项目(200705C05)。

In vitro Culture and Efficient Plantlet Regeneration of Rhododendron anthopogonoides Maxim.

GU Di-Zhou*, LU Shuang, BA Chun-Ying, LI Yuan-Yuan

Department of Biology, Tonghua Normal University, Tonghua, Jilin 134002, China

Corresponding author: GU Di-Zhou; E-mail: gudizhou@163.com; Tel: 0435-3208073

Abstract:

The tender stems of Rhododendron anthopogonoides were used as explants in the experiment, the uniform design was used to screen the most suitable media for shoots regeneration by the tender stems and rooting. The results showed that the best medium for shoots regeneration was DR+2.30 mg·L^-1 TDZ+0.05 mg·L^-1 IAA+1.80 mg·L^-1 KT, the rate of induction was 99.6%; the best medium for rooting was 1/2DR+0.07 mg·L^-1 IAA+0.02 mg·L^-1 NAA, the rate of rooting was 99.7%. Each stems with one node were cut from regenerated shoots and cultured for propagation, and proliferation rate achieved an average of 5 within 35 days.

Key words: Rhododendron anthopogonoides; in vitro culture; plantlet regeneration; uniform design